FastQCFastQC Report
Mon 7 Oct 2024
quad_209_S20_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamequad_209_S20_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59219751
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12174292.0557820312348154No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC1417780.23940999008928626No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT976680.16492470561046432No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC923480.15594121630129787No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC891150.15048188905758825No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG890120.15030796059915888No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT783090.13223459855479636No Hit
CCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCC735950.12427441648648607No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC719430.12148480664837648No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT694150.11721596060071242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACG323250.030.4069166
TGGCGCA323500.030.134014
CGGTGGC391050.026.6034951
GGTGGCG411200.024.9651532
GGCGCAC396300.024.8499095
CGCGGTG92250.024.1288641
GTGGCGC424000.024.0995523
CGCACGC485500.020.7534921
TCCCACG721300.019.9434742
GCACGCC523650.019.3049772
ACGTCCG750300.019.2801676
CCCACGT763450.018.6807653
CGTCCGG779000.018.4845527
CACGCCT557400.018.0935083
CACGTCC796700.018.0083585
CCACGTC797700.017.866794
GTCCGGG847750.017.2568668
ACGCCTG622500.016.1022054
CTCCCAC997150.015.9541611
GCCCTCT619150.015.054853
Produced by FastQC (version 0.11.4)