FastQCFastQC Report
Mon 7 Oct 2024
quad_208_S19_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamequad_208_S19_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60295449
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12437752.062800792809421No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC1380250.22891445754056827No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG941580.15616103961677108No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC873950.14494460435977516No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT870490.14437076337220742No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC855540.14189130592592486No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT770810.12783883573037164No Hit
CCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCC718960.11923951341667595No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC690690.11455093401825402No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT669850.11109462009313506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACG328450.030.7923166
TGGCGCA342850.029.4297984
CGGTGGC407900.026.8307231
CGCGGTG93800.024.843081
GGTGGCG437400.024.6523172
GGCGCAC420650.024.1785055
GTGGCGC455500.023.5268483
CGCACGC478600.021.4591627
GCACGCC519600.019.6805698
TCCCACG711850.019.5413912
ACGTCCG732800.019.0928256
CACGCCT550800.018.790439
CCCACGT749300.018.4570393
CGTCCGG761400.018.4192877
CCACGTC776650.017.8620644
CACGTCC781800.017.8172475
GTCCGGG817600.017.3926938
CTCCCAC996300.015.7008751
GCCCTCT627300.014.5943633
ACGCCTG621950.014.3383614
Produced by FastQC (version 0.11.4)