FastQCFastQC Report
Mon 7 Oct 2024
quad_208_S19_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamequad_208_S19_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60295449
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGG10113791.6773720351597348TruSeq Adapter, Index 3 (97% over 37bp)
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG2011100.3335409277738358No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1348310.22361720865533316No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1010490.1675897628691678No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT938120.15558719862920334No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG634480.10522850572022442No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT604160.10019993382916843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2059800.053.5823639
AGAGCAC2065850.053.4286778
ATCGGAA2221350.049.779582
CGGAAGA2253750.049.1443944
GATCGGA2286500.048.2876471
TCGGAAG2331300.047.580923
AAGAGCA2336550.047.4594887
TCGCTAT234150.046.801782
CTCGCTA247300.044.8428761
GAAGAGC2538950.043.6461566
CGCTATG257450.042.5463033
ACGTAGA1292700.041.4305232-33
GTCACGT1365400.040.75812528-29
CGTAGAG1362150.040.5578632-33
TCACGTA1330150.040.42875330-31
AGTCACG1382300.038.90494528-29
TAGAGAT1511750.036.81405334-35
CGTCTGA1523800.035.5676916-17
CACGTAG1565300.035.4686630-31
GAGATCT1605550.034.542636-37
Produced by FastQC (version 0.11.4)