FastQCFastQC Report
Mon 7 Oct 2024
quad_207_S15_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamequad_207_S15_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60656979
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37081856.113369081569328No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC1915740.3158317528474341No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC1311030.21613836059985775No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC1167680.19250546585908934No Hit
CCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCC1140470.18801958468785596No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG772910.1274230950407207No Hit
CAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAAAA702030.11573771255571433No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT701330.11562230951198543No Hit
CCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCC689990.11375278020357724No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC652360.10754904229569363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCA295350.028.892524
GCGCACG298500.028.7466226
CGGTGGC353100.026.452011
CGCGGTG84100.023.758411
CGCACGC416800.020.735527
CCTTATA233550.019.3865816
CACGCCT462650.018.9120359
CGATACG41150.018.6843456
GCACGCC473600.018.4245628
GGCCTTA254750.018.350794
GCCCTCT745450.018.2596873
CGGAACG143600.018.1446741
TCCCACG1097150.017.9434152
CAACCCG205300.017.9240389
CCCACGT1113150.017.3401643
GCCTTAT269300.017.1654555
ACGTCCG1140850.017.0772276
CCACGTC1157900.016.665914
CACGTCC1164800.016.66095
CGAGGCG152750.016.6227871
Produced by FastQC (version 0.11.4)