FastQCFastQC Report
Mon 7 Oct 2024
quad_207_S15_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamequad_207_S15_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60656979
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTGGGGGGG31957685.268590775020298TruSeq Adapter, Index 13 (97% over 43bp)
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA1943550.32041655091329224No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT1705320.28114159790252663No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG1364270.22491558638289585No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGC1102820.18181254955015153No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT1069860.17637871480543071No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA1063640.1753532763311539No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA954110.1572959972174018No Hit
CTAACAATGAATTTTCACATATAAGTTGGATTTTAATTCTATTTATTTAT910330.15007836113961429No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA796090.13124458440305772No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT770530.12703072469204243No Hit
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG741320.12221512053872646No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC734930.12116165561097264No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT666360.10985710317027164No Hit
CTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAA617870.10186296946967964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA4593050.073.97789
AGAGCAC4619050.073.577288
CGGAAGA4849900.069.9633564
AAGAGCA4870650.069.866947
ATCGGAA4918800.068.990582
TCGGAAG4969100.068.401733
GATCGGA4981850.067.9809951
GAAGAGC5327300.063.826186
TCGCTAT158750.050.9652982
GGAAGAG6998900.048.8272325
CTCGCTA168850.048.3313561
CGCTATG171200.047.091173
GTCAAAT3848500.044.40131434-35
CAGTCAA3861950.044.12670532-33
AATCTGG3903550.043.86074438-39
CAAATCT3938100.043.29003536-37
TCAAATC3992400.042.0218936-37
CGTCTGA4034100.041.96545816-17
AAATCTG4019750.041.6914738-39
ACGTCTG4101800.041.6365814-15
Produced by FastQC (version 0.11.4)