FastQCFastQC Report
Mon 7 Oct 2024
quad_206_S14_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamequad_206_S14_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52143710
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36335246.968288217313267No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC1336870.25638183397383885No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC958680.18385343121922088No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC819860.157230852963857No Hit
CCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCC797230.1528909239484494No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG776080.14883482590709407No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT647170.1241127645117695No Hit
CAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAAAA588750.11290911214411097No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC584270.11204994811454727No Hit
CAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAAC559840.10736481926583283No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT532950.10220791731159905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCA266900.030.6205124
GCGCACG271300.030.2288636
CGGTGGC305150.028.83521
CGCGGTG71300.024.3622931
CGATACG45000.023.5190756
CGCACGC370800.022.3092787
CACGCCT418100.020.119589
GCACGCC414150.020.0930888
CAACCCG204950.019.9297529
CGGAACG123250.019.6385691
CCTTATA199500.018.9364266
GATTCAA254350.018.2674875
GTTATAC251300.018.2625685
GAACGAT62200.017.3206163
GGCCTTA228450.016.8483564
GCCCTCT601800.016.8373433
GCCTTAT232800.016.5946965
GGATTCA298650.016.1934554
CGAGGCG138750.016.0422941
CCCACGT818200.015.9862873
Produced by FastQC (version 0.11.4)