FastQCFastQC Report
Mon 7 Oct 2024
quad_205_S18_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamequad_205_S18_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62580546
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20452973.268263271464586No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC1327390.21210904743464526No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT1006970.16090783228385383No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG1002000.16011365576772055No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC910940.14556280796910911No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT763030.1219276674255926No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC762870.12190210037477142No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT729650.11659374144802125No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC675350.1079169235755789No Hit
CCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCC662100.10579965217944888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACG347200.030.0727546
TGGCGCA346300.029.9727444
CGGTGGC442900.025.1070771
GGCGCAC430600.024.2371445
CGCGGTG101600.024.0103741
GGTGGCG483250.022.8339462
GTGGCGC489850.022.3325373
CGCACGC512400.020.4512637
TCCCACG672850.019.4397582
ACGTCCG690650.019.0487046
GCACGCC557500.018.7775618
CCCACGT707550.018.3857823
CACGCCT577950.018.2613939
CACGTCC732700.017.949015
CGGAACG49200.017.9422681
CGTCCGG747300.017.6745537
CCACGTC740000.017.6372624
GTCCGGG763950.017.5479558
ACGCCTG659300.015.32578854
CTCCCAC961250.015.2366361
Produced by FastQC (version 0.11.4)