FastQCFastQC Report
Mon 7 Oct 2024
quad_204_S13_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamequad_204_S13_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57521809
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33117365.757357179083154No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC2123420.36915042084298844No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC1520100.26426498512937935No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC1324950.23033872248350187No Hit
CCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCC1201170.20881992775992148No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG1023680.17796380499785744No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT869640.15118439686067592No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC779350.13548774170158662No Hit
CAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAAAA752040.13073997724932468No Hit
CCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCC742750.12912493763887017No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT698270.12139221838450873No Hit
CAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAAC626620.1089360732726608No Hit
ATTAAAGTAAGCAAAAGAATCAAACATAAAAACGTTAGGTCAAGGTGTAG619070.10762352762584361No Hit
AAAGAATCAAACATAAAAACGTTAGGTCAAGGTGTAGCCAATGAAATGGG604680.10512186777714172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCA339050.031.7334164
GCGCACG347300.031.1709186
CGGTGGC375300.031.0204181
CGCGGTG87800.028.7030931
GGTGGCG505500.022.899172
CGCACGC477100.022.8298137
GTGGCGC518300.022.1962953
CCTTATA259600.021.9018696
GGCGCAC504350.021.5869085
GGCCTTA271000.021.0155644
GCCCTCT766900.020.9668773
GCACGCC530950.020.6392298
CACGCCT543150.020.3421449
GCCTTAT293150.019.6057435
TCCCACG1101400.019.0116372
CGGAACG97750.018.6441751
CGAGGCG155750.018.618381
CCCACGT1199800.017.3021073
ACGTCCG1225250.017.117036
CGACAAT182300.017.0783461
Produced by FastQC (version 0.11.4)