FastQCFastQC Report
Mon 7 Oct 2024
quad_203_S12_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamequad_203_S12_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56159823
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41385197.36918098904977No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC1737880.3094525422560538No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC1413450.2516834855409No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG1110570.1977516916319341No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC1058480.18847637749855442No Hit
CCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCC1006880.17928831435241524No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT836280.14891072573359074No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC770360.13717279700115864No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT715700.1274398603428647No Hit
CCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCC609390.10850995737646822No Hit
CAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAAC595960.10611856807312232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCA362850.032.712594
CGGTGGC394400.032.2978171
GCGCACG370550.032.0968936
CGCGGTG88350.026.8590741
GGCGCAC457800.026.0937065
GGTGGCG485650.026.014352
GTGGCGC491950.025.7101483
CGCACGC500600.023.815387
GCACGCC543850.021.9890278
CACGCCT579200.020.7373589
GCCCTCT712950.020.143653
TCCCACG951550.018.7236712
ACGTCCG1025700.017.4395186
CCCACGT1028200.017.2401623
CCTTATA215050.016.8389036
CACGTCC1071050.016.67455
CCACGTC1068750.016.6482124
CTCCCAC1168050.016.1795851
GTCCGGG1131000.016.0413868
CGTCCGG1121400.015.9554667
Produced by FastQC (version 0.11.4)