FastQCFastQC Report
Mon 7 Oct 2024
KB_untrtd_S10_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameKB_untrtd_S10_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58324116
Sequences flagged as poor quality0
Sequence length101
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51578888.843491086945923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC104000.030.533099
AGTTCCG114150.027.4408747
GTTCCGA119450.026.105638
TCCGATC96800.024.7596824-25
TCCGACG69550.022.28085110-11
CGATCAG92350.020.71086526-27
TTCCGAT109350.019.16200424-25
GACGTGT144800.018.75310712-13
CCGACGT88900.018.10059410-11
TCAATCG79200.017.138423
TGGCGCG260900.015.9170983
GCGACAT102050.014.8821927
GACTGGA399000.014.5354031
CCGATCA115550.014.31406326-27
CGGGATA116600.014.1648054
CAATCGC92250.014.096594
CGATCCG35600.013.66482226-27
GGCGATC93150.013.2980252
TCTTCCG187550.013.15877422-23
ACGTGTG194500.012.94364514-15
Produced by FastQC (version 0.11.4)