FastQCFastQC Report
Mon 7 Oct 2024
KB_untrtd_S10_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameKB_untrtd_S10_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58324116
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTGGGGGGG44297067.594981808211204TruSeq Adapter, Index 7 (97% over 36bp)
CGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG915910.1570379566490129No Hit
CCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG757490.1298759504558972No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT602650.10332775553769215No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT593860.10182066025655664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA6106700.078.508369
AGAGCAC6172700.077.71018
CGGAAGA6266900.076.457754
AAGAGCA6358850.075.458347
ATCGGAA6363500.075.2923662
TCGGAAG6390650.075.10173
GATCGGA6405800.074.790531
GAAGAGC6412850.074.765416
GGAAGAG6922950.069.313715
CGAGTGG5143400.045.6546632-33
CACGAGT5179050.045.39736630-31
ACGAGTG5134850.045.3845232-33
GTCACGA5261150.044.92246628-29
AGTGGAT5300400.044.690634-35
AGTCACG5288650.044.2844928-29
TCACGAG5283950.044.26135630-31
TGGATCT5373250.044.03736-37
GATCTGG5439750.043.69739538-39
GTGGATC5380550.043.42656336-37
CAGTCAC5498350.043.0963626-27
Produced by FastQC (version 0.11.4)