FastQCFastQC Report
Mon 7 Oct 2024
KB_trtd_S11_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameKB_trtd_S11_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60824890
Sequences flagged as poor quality0
Sequence length101
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52973418.7091665928208No Hit
GGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGAT783530.12881733119451594No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGCGGGGAATCC699050.11492828018266864No Hit
CGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCC640460.10529571035804586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC99250.024.84173224-25
TTCCGAC145850.023.4363049
AGTTCCG149750.022.0265837
TCCGACG75650.020.57831210-11
GACGTGT137000.019.71605712-13
CCGACGT82900.019.20934310-11
GTTCCGA194350.017.1959238
TTAACGG52200.016.274653
GACTGGA369750.015.69800951
TTCCGAT139900.015.33372124-25
GGCGATC102350.015.1631012
ATTAACG56200.015.1162772
GCGACAT101250.014.6247617
CGATCAG133500.014.575726-27
CGATCCG34200.014.2934826-27
ACGTGTG179050.013.98046414-15
GATTCAA640500.013.9305385
ATTCAAC642600.013.7003726
CAACCCG690000.013.68874559
CGATCTG118850.013.6170914
Produced by FastQC (version 0.11.4)