FastQCFastQC Report
Mon 7 Oct 2024
KB_trtd_S11_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameKB_trtd_S11_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60824890
Sequences flagged as poor quality0
Sequence length101
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTGGGGGGG44920677.385244757532648TruSeq Adapter, Index 22 (97% over 37bp)
CTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGA1979090.3253750232840536No Hit
CGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG1056790.17374301868856648No Hit
CCCGTCGCCGGGGCGGGGGCGCGGGGAGGAGGGGTGGGAGAGCGGTCGCG898620.14773886150883297No Hit
CCTGCCGCCCCGACCCTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCC898260.1476796752119075No Hit
CCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG891900.1466340506328906No Hit
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG864320.142099722662877No Hit
GGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAG789160.1297429391158784No Hit
GGCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCTGCCGC765270.125815270689351No Hit
CGGTCCCGCCGCCCCCGCCGCCGCCGCCACCGCCGCCGCCGCCGCCGCCC739460.1215719420125544No Hit
GGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCGCCGCC739340.12155221324691257No Hit
CTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCC732980.12050658866789565No Hit
CCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG704580.11583744746599624No Hit
CCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGAAGGG661960.10883044753554014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA6665900.074.173059
AGAGCAC6769000.073.042718
AAGAGCA7019250.070.494597
CGGAAGA7027200.070.218474
GAAGAGC7084500.069.780766
TCGGAAG7195800.068.702173
ATCGGAA7248350.068.111212
GATCGGA7309050.067.514661
GGAAGAG7665050.064.54135
CGGTAGC5352300.045.32732832-33
ACGGTAG5371350.044.95667332-33
GTAGCAT5425450.044.91992634-35
CACGGTA5427250.044.75944530-31
TAGCATC5431000.044.40373236-37
GTCACGG5533250.044.15849328-29
GGTAGCA5443800.044.1495734-35
AGTCACG5541800.043.791428-29
TCACGGT5552350.043.69479830-31
ATCTGGG5567150.043.28457640-41
AGCATCT5627050.043.27038236-37
Produced by FastQC (version 0.11.4)