FastQCFastQC Report
Mon 7 Oct 2024
DM_105_untrtd_S8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDM_105_untrtd_S8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50225038
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44097408.779963491515923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGA79750.023.8197428
TCCGATC75000.020.94552424-25
TGGCGCG240000.019.4585023
AGTTCCG100700.019.323267
TTCCGAC98550.019.2273549
TCAATCG72400.018.3546053
TCCGACG56150.016.19484110-11
TTCCGAT82400.016.12706624-25
CAATCGC93800.014.7236584
CGCGTGC439400.014.5705421
GTGGCGC333200.014.3148442
CGATCAG89550.014.203444526-27
GCGTGCC451450.013.6455362
GACGTGT132650.013.03085512-13
CCGACGT77350.012.8025410-11
GGCGCGT393200.012.6857114
AATCGCC114700.012.2476815
CCGATCA88500.012.0660126-27
GGGCGAT67850.011.8211761
GCGACAT87850.011.3989987
Produced by FastQC (version 0.11.4)