FastQCFastQC Report
Mon 7 Oct 2024
DM_105_untrtd_S8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDM_105_untrtd_S8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50225038
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTGGGGGGG38104367.5867259672357035TruSeq Adapter, Index 21 (97% over 43bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT662540.1319142854605705No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA519460.10342650213624527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA4937050.083.515331
CGGAAGA4941500.083.454714
ATCGGAA4940800.083.450262
GAGCACA4989000.082.749999
AGAGCAC5003600.082.517048
TCGGAAG5013200.082.426263
AAGAGCA5153100.080.176337
GAAGAGC5151400.080.169396
GGAAGAG5502350.075.118395
CGTTTCG4371350.046.51553732-33
TTTCGAT4453700.045.9046734-35
GTTTCGA4407250.045.7130134-35
TTCGATC4419400.045.7036936-37
ACGTTTC4467050.045.3090832-33
GTCACGT4525600.045.27932428-29
CACGTTT4503050.045.25003430-31
TCGATCT4523450.045.03661336-37
TCACGTT4530150.044.908430-31
CGATCTG4496100.044.90128738-39
AGTCACG4528600.044.866228-29
Produced by FastQC (version 0.11.4)