FastQCFastQC Report
Mon 7 Oct 2024
DM_105_trtd_S9_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDM_105_trtd_S9_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60658986
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56454229.306818943528004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGA102850.029.3472848
AGTTCCG116500.025.8277997
TTCCGAC122650.024.7648129
TCCGATC100550.024.07193224-25
TCCGACG73200.020.12941210-11
TTCCGAT106200.019.57367924-25
TCAATCG79900.018.1760183
TGGCGCG275100.017.9418353
GACGTGT164600.016.32393512-13
CGATCAG120650.016.0493126-27
CCGACGT98750.015.78904110-11
CCGATCA113800.014.34629426-27
CAATCGC104050.014.1854264
GTGGCGC381850.013.1372942
CGCGTGC489150.013.10798551
GGCGCGT423900.012.8641394
GCGTGCC512100.012.2425472
AATCGCC127550.012.1672215
GGCGATC85400.012.0593982
TCTTCCG194000.011.92602222-23
Produced by FastQC (version 0.11.4)