FastQCFastQC Report
Mon 7 Oct 2024
DM_103_untrtd_S6_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDM_103_untrtd_S6_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63012765
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG645826010.2491296803116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC112800.024.76100224-25
TCCGACG80750.020.95834710-11
GTTCCGA181350.020.44948
AGTTCCG179300.020.4082437
TTCCGAC186250.020.0391719
TGGCGCG310300.018.8740433
CCGACGT102300.017.00872210-11
GACGTGT186400.016.51103412-13
TTCCGAT156050.015.79983724-25
CCGATCA131550.015.26090226-27
GGGCGAT85000.014.9639651
GCGACAT115000.014.4444747
CAACCCG250150.014.3683359
GTGGCGC418100.014.1893132
TCAATCG72100.014.0867263
GGCGATC93300.014.0397382
ATTAAGC243200.013.66048056
GACTGGA482400.013.4392251
GGCGCGT470450.013.38717654
TCTTCCG199350.013.36793522-23
Produced by FastQC (version 0.11.4)