FastQCFastQC Report
Mon 7 Oct 2024
DM_103_trtd_S7_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDM_103_trtd_S7_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59525284
Sequences flagged as poor quality0
Sequence length101
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG658486211.062294133699556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGA135100.025.3713578
TCCGATC109600.025.0506324-25
AGTTCCG144750.023.6069937
TCAATCG84100.023.5888863
TTCCGAC151000.022.9204399
TCCGACG78550.021.57590710-11
TGGCGCG391750.020.9950393
TTCCGAT115850.019.80734824-25
CAATCGC105700.019.127644
GGGCGAT78250.016.8610941
CCGACGT106050.016.2279210-11
GTGGCGC520300.016.0996822
AATCGCC134450.016.0258775
CCGATCA117400.015.4426526-27
GGCGCGT603550.014.854074
CGATCAG150850.014.72404526-27
GACGTGT209250.014.67417912-13
GGCGATC97400.014.0820312
ATCAATC160400.013.5515042
CGCGTGC665000.013.4386446
Produced by FastQC (version 0.11.4)