FastQCFastQC Report
Mon 7 Oct 2024
DM_103_trtd_S7_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDM_103_trtd_S7_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59525284
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGG56833709.547825088915157TruSeq Adapter, Index 20 (97% over 43bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1123550.1887517243932847No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT940920.15807064440045343No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT900260.15123993360535667No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG825020.13859992671349539No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA772620.12979694477392162No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG609190.10234138488108684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA7742150.080.068899
AGAGCAC7818900.079.261338
CGGAAGA7962150.077.86064
AAGAGCA8019650.077.3180167
ATCGGAA8063850.076.8120652
GAAGAGC8097850.076.492726
TCGGAAG8118000.076.4668663
GATCGGA8113000.076.3357241
GGAAGAG8540500.072.69415
GTCACGT6816300.045.1384728-29
CGTGGCC6746350.045.1163632-33
ACGTGGC6747800.044.8784332-33
TCACGTG6811750.044.6484930-31
AGTCACG6846350.044.41404728-29
CACGTGG6887800.044.3508230-31
GGCCATC6912200.043.5776336-37
TGGCCAT7035900.043.4602334-35
CAGTCAC7093250.043.4059226-27
CGTCTGA7141500.043.19465316-17
TCCAGTC7247500.043.14782724-25
Produced by FastQC (version 0.11.4)