FastQCFastQC Report
Mon 7 Oct 2024
DM_102_untrtd_S4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDM_102_untrtd_S4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60041805
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG648230610.796320996678897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGA121700.025.5839358
AGTTCCG123100.025.1372177
TCCGATC102300.023.56757724-25
TCAATCG92600.023.4224593
TTCCGAC141300.022.5068369
TCCGACG69450.021.76463910-11
TGGCGCG319850.019.5122183
CAATCGC116050.018.4441494
TTCCGAT111700.017.97273324-25
GGGCGAT67450.017.6611081
CCGACGT97600.016.24330510-11
GACGTGT170600.016.08495112-13
GCGACAT104700.015.7746657
GTGGCGC413000.015.4445682
AATCGCC148000.015.29622655
GGCGCGT450450.015.0771864
CGATCAG138650.014.70179626-27
CGCGTGC569250.014.4818131
GCGTGCC591150.013.5840472
CCGATCA118950.013.48588126-27
Produced by FastQC (version 0.11.4)