FastQCFastQC Report
Mon 7 Oct 2024
DM_102_trtd_S5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDM_102_trtd_S5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56392608
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG625222211.086953098533765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC102000.024.73025524-25
AGTTCCG133600.024.404857
GTTCCGA137750.024.0882078
TTCCGAC154250.021.9435759
TCCGACG72100.021.6571510-11
TCAATCG87900.019.4914533
TGGCGCG300100.018.6771143
TTCCGAT114850.018.59546324-25
GGGCGAT76350.017.342841
GACGTGT163950.016.9399812-13
CCGACGT96900.016.75302910-11
GGCGATC85850.015.7554232
CAATCGC110550.015.4550224
GCGACAT104600.014.7461137
GTGGCGC401100.014.1397182
CGATCAG145750.013.96928726-27
GACTGGA435900.013.6750171
CGATCCG36950.013.35809126-27
CCGATCA125400.013.28418926-27
CGCGTGC549950.013.1087011
Produced by FastQC (version 0.11.4)