FastQCFastQC Report
Mon 7 Oct 2024
DM_102_trtd_S5_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDM_102_trtd_S5_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56392608
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTGGGGGGG53767359.534467708959301TruSeq Adapter, Index 18 (97% over 45bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT893770.158490630545053No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT833030.1477197153215542No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT755690.134005151880899No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG723660.12832532944743397No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT636660.11289777553824076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA7251000.081.0489659
AGAGCAC7333250.080.2105568
CGGAAGA7424100.079.279834
ATCGGAA7537100.078.100662
TCGGAAG7553800.078.0570453
AAGAGCA7546400.078.049217
GATCGGA7570750.077.763461
GAAGAGC7572150.077.697596
GGAAGAG7969950.073.915365
TCCGCAT6354150.046.01151734-35
CGCATCT6363100.045.7122336-37
CGTCCGC6395950.045.5168932-33
GTCACGT6476050.045.18505528-29
ACGTCCG6336950.045.1677332-33
CCGCATC6361350.044.9182236-37
GTCCGCA6362100.044.79710834-35
CACGTCC6546050.044.55535530-31
AGTCACG6492900.044.1604328-29
TCACGTC6536550.043.99580830-31
CAGTCAC6723600.043.5583326-27
Produced by FastQC (version 0.11.4)