FastQCFastQC Report
Mon 7 Oct 2024
Ctrl_203_untrtd_S2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCtrl_203_untrtd_S2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61943382
Sequences flagged as poor quality0
Sequence length101
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1128861018.224077593955073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGA131500.028.7094548
AGTTCCG135250.027.6863147
TCCGATC114400.025.3684924-25
TTCCGAC155300.024.432269
TCCGACG83150.021.6710410-11
GGGCGAT83100.021.3025441
TGGCGCG286450.020.5114753
GGCGATC87000.020.4567222
TTCCGAT124750.019.78276324-25
GACGTGT169050.019.32078412-13
CCGACGT108250.017.54773710-11
TCAATCG78600.016.6652413
GACTGGA430900.016.4549961
CGATCAG147550.016.24338326-27
CCGATCA125600.015.87025826-27
GTGGCGC385600.015.4834462
GCGACAT132950.014.9572277
GGCGCGT435050.014.5199114
CGATCCG37600.014.01068926-27
TGGAGTT517050.014.0070614
Produced by FastQC (version 0.11.4)