FastQCFastQC Report
Mon 7 Oct 2024
Ctrl_201_untrtd_S1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCtrl_201_untrtd_S1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80204151
Sequences flagged as poor quality0
Sequence length101
%GC76

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2474463730.852065250338477No Hit
CGGCGGTCGGCGGGCGGCGGGGCGGGGCGGTTCGTCCCCCCGCCCTACCC1517560.18921215187478266No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGCGGTTCGTCCCCCCGC1306930.16295041886298378No Hit
CGGCGGGCGGCGGGGCGGGGCGGTTCGTCCCCCCGCCCTACCCCCCCGGC922990.11508007858595747No Hit
CCCCTCGCGGCCCTCCCCCGCCCCACCCCGCGCGCGCCGCTCGCTCCCTC892470.11127478925623188No Hit
GCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGCGGTTCGTCCCCCCGCCCT873500.10890957501688411No Hit
CGGGGAGCGGTCGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGCGGTT830120.10350087740471188No Hit
CCCCACGCCCGGGGCACCCCCCTCGCGGCCCTCCCCCGCCCCACCCCGCG820350.10228273596462607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGGA530600.045.1972121
TGGAGTT537700.043.6119234
ACTGGAG601350.038.940542
AGTTCCG351750.038.0356987
TTCCGAC377950.036.601739
TCCGACG182500.035.37014810-11
ATCCGAT54400.034.7664428-29
GTTCCGA384850.034.7209668
TCCGATC293850.034.6604124-25
CCGACGT185450.034.64065610-11
AGTTCAG299850.032.463257
CGATCCG65700.030.737226-27
GACGTGT369500.030.63085612-13
TAGCATA88500.028.7773519
ACGTGTG371650.026.46392614-15
TCAGACG181250.025.72565710-11
CAGACGT196150.025.15481210-11
TTCAGAC392300.024.3054129
TCAATCG136400.024.182483
TCTTCCG415550.024.10985222-23
Produced by FastQC (version 0.11.4)