FastQCFastQC Report
Mon 7 Oct 2024
Ctrl_201_untrtd_S1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCtrl_201_untrtd_S1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80204151
Sequences flagged as poor quality0
Sequence length101
%GC70

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTGGGGGGG2105681026.254015206769036TruSeq Adapter, Index 4 (97% over 45bp)
CCTGCCGCCCCGACCCTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCC3697940.4610659116633502No Hit
GGCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCTGCCGC3123160.3894012917111983No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCTGCCGCC2753910.34336252745821094No Hit
CCCGTCGCCGGGGCGGGGGCGCGGGGAGGAGGGGTGGGAGAGCGGTCGCG2653800.3308806298566766No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAGATCGGAAGAGCACACGT2002900.24972522930889202TruSeq Adapter, Index 1 (97% over 36bp)
CTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGA1769830.220665636121502No Hit
TCCCCCGCCGACCCCACCCCCGGCCCCGCCCGCCCACCCCCGCACCCGCC1667360.20788948941059174No Hit
CCCACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACAC1561240.1946582540347569No Hit
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG1374560.17138265075581935No Hit
CCCCCGCCGACCCCACCCCCGGCCCCGCCCGCCCACCCCCGCACCCGCCG1237440.15428627877377568No Hit
CTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAG1184930.14773923609016196No Hit
GATCGAAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTGGGGGGG1059320.13207795192545582TruSeq Adapter, Index 4 (97% over 43bp)
GGGGGACCGGCCCGCGGCCCCTCCGCCGCCTGCCGCCGCCGCCGCCGCGC924020.11520850086674442No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT867650.1081801863347447No Hit
CGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCTGCCGCCG840830.10483621976124402No Hit
CGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCTGCCGCCGCCGCCG813600.10144113363908061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA27175050.085.67399
AGAGCAC27441900.084.836648
AAGAGCA27673750.084.214057
CGGAAGA27673800.083.508714
GAAGAGC27830400.083.346776
GATCGGA27913850.082.8641361
ATCGGAA28027950.082.580332
TCGGAAG28046350.082.521973
GGAAGAG28569250.081.075235
GATCGAA167500.076.879311
ATCGAAA203750.058.071142
AATCGGA117400.057.2422751
TCGGAAA166950.049.4355433
GACCAAT24965250.046.31270234-35
CCAATCT24992800.046.2727836-37
AATCTGG25269250.045.9925638-39
ACCAATC24989600.045.9577336-37
CTGACCA25114100.045.90828732-33
ACTGACC25110050.045.71429432-33
CACTGAC25221600.045.70882830-31
Produced by FastQC (version 0.11.4)