FastQCFastQC Report
Thu 8 Feb 2024
dm_105_untreated_S13_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamedm_105_untreated_S13_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49415492
Sequences flagged as poor quality0
Sequence length101
%GC85

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3565504772.15358090535656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG99950.042.0102773
TCAATCG22800.036.249373
GTGGCGC120750.035.245942
GGCGCGT123850.034.7083744
CGCCGTA17050.030.50697358-59
CGCGTGC143250.030.1405356
CAATCGC27100.029.796234
GCGTGCC157400.027.5818357
GGGCGAT21350.026.9200421
GGTGGCG170950.025.646071
CGTGCCT173000.025.040568
GCGCGTG172350.024.9688535
AATCGCC37550.023.4014875
GGCGATC26100.022.3847832
AGGGGGG128300.022.213291
GCCGTAT24300.020.32999860-61
TAGGCGA39000.019.73049532-33
TGGTCGC40100.018.41991854-55
CGTATCA25400.017.95343462-63
ATCGCCC49800.016.9774466
Produced by FastQC (version 0.11.4)