FastQCFastQC Report
Thu 8 Feb 2024
dm_105_untreated_S13_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamedm_105_untreated_S13_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49415492
Sequences flagged as poor quality0
Sequence length101
%GC69

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGGGGG2837651257.424323529956965TruSeq Adapter, Index 13 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTGGGGGGG34659617.013915797904026TruSeq Adapter, Index 13 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGGTGG7934971.6057656574581913TruSeq Adapter, Index 13 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGGGGC1975710.3998159119816109TruSeq Adapter, Index 13 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTGGGGTGG1016530.20571079207306078TruSeq Adapter, Index 13 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGTGGG966540.19559453136680297TruSeq Adapter, Index 13 (97% over 45bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGGGGGG917170.1856037373866479TruSeq Adapter, Index 13 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGGGTG775540.15694268509964446TruSeq Adapter, Index 13 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGGGGT639090.12932988707266135TruSeq Adapter, Index 13 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA35302000.094.811791
ATCGGAA35422050.094.347142
TCGGAAG35370450.094.3141863
CGGAAGA35584350.093.67674
AAGAGCA35701700.093.4204947
GAAGAGC35693150.093.39426
AGAGCAC35714700.093.299328
GAGCACA35684900.093.293839
GGAAGAG35812650.093.1610265
ATCGGAG121400.082.302862
GATCGAA62550.081.47541
TCGGAGA117150.075.820443
TCGGAAA85700.070.4539643
ATCGAAA44050.065.008222
TCGAAAG40600.063.8199043
CGGAGAG141100.062.868564
CGAAAGA42500.060.975444
CGGAAAG70600.059.29464
GATCGTA18550.059.2762261
TATCGGA22650.058.466651
Produced by FastQC (version 0.11.4)