FastQCFastQC Report
Thu 8 Feb 2024
dm_105_treated_S14_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamedm_105_treated_S14_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32961913
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18074905.4835712963625625No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA337110.102272583511764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG45700.029.6220513
TGGCGCG210850.026.2445283
CAATCGC58400.024.2373564
GTGGCGC268650.020.8632642
GGCGCGT288300.020.5448824
AATCGCC72300.019.5776165
CGCGTGC325400.018.0419226
GCGTGCC348050.016.4720427
GCGCGTG388150.015.3699295
GGTGGCG368600.015.2446171
CGTGCCT396750.014.6539118
ATCAATC108200.014.4429442
ATCGCCC108500.013.6148386
CGGTGGC302150.013.1737231
GATCAAT124700.012.569981
AGTGCGT89550.012.01404930-31
GGCGATC47200.011.8748222
GGGCGAT47500.010.9998351
GGTCGAC22950.010.7622365
CTACTCG509050.010.7491436
Produced by FastQC (version 0.11.4)