FastQCFastQC Report
Thu 8 Feb 2024
dm_105_treated_S14_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamedm_105_treated_S14_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32961913
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC12757863.870485308301129TruSeq Adapter, Index 14 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTGGTATGC1354820.41102590131828814TruSeq Adapter, Index 14 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTGTGC912820.27693174240220825TruSeq Adapter, Index 14 (97% over 46bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT516710.15675971233829783No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA513120.15567057652266725No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT468270.1420639633385356No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC403480.12240794398067854No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT399240.1211216108725243No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG347670.10547628106414818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2375550.069.1439069
AGAGCAC2403650.068.440238
CGGAAGA2481650.066.610534
ATCGGAA2539550.065.32932
AAGAGCA2538000.064.970687
TCGGAAG2555100.064.867393
GATCGGA2584500.064.2961041
GAAGAGC2572700.064.229046
GGAAGAG2830700.058.691895
TCGTATG1416050.046.24585342-43
ATGCCGT1515900.045.26841446-47
TATGCCG1514300.045.23945246-47
TCTCGTA1460000.045.11217540-41
GTATGCC1565150.045.08885244-45
GCCGTCT1388700.044.56820348-49
CGTATGC1408750.044.4274944-45
CATCTCG1692650.044.01376338-39
ATCTCGT1595900.043.89529440-41
TGCCGTC1538700.043.1339348-49
CTCGTAT1520200.042.2048542-43
Produced by FastQC (version 0.11.4)