FastQCFastQC Report
Thu 8 Feb 2024
dm_104_untreated_S11_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamedm_104_untreated_S11_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34249333
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18193885.31218520372353No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA369420.10786195456711523No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT358050.10454218188716258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG61400.034.889533
CAATCGC82450.025.9818254
TGGCGCG266450.024.7256493
AATCGCC97100.022.795575
GTGGCGC333050.020.1236062
ATCAATC118700.018.8477042
GATCAAT134700.017.0673451
GGCGCGT432650.016.7094334
GCGTGCC447900.015.29212957
CGCGTGC473600.015.2746756
ATCGCCC156350.014.7646166
CGTGCCT508550.013.7115838
GGTGGCG528350.012.9817631
GCGCGTG615900.011.9692055
CGGAGGG119700.011.8252511
GGGCGAT43600.011.6569661
TAGTCCC500400.011.09162516-17
TCGCCCC207650.011.0483897
CGGTGGC449850.010.9601831
GGCGATC46050.010.8304842
Produced by FastQC (version 0.11.4)