dm_104_untreated_S11_R1_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	dm_104_untreated_S11_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34249333
Sequences flagged as poor quality	0
Sequence length	101
%GC	51

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC	1443454	4.214546309558788	TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTATGC	140339	0.40975688490050305	TruSeq Adapter, Index 2 (98% over 50bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA	67008	0.1956476057504536	No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT	53010	0.15477673681995502	No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC	46632	0.13615447635140807	No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT	40013	0.11682855254436635	No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG	38999	0.11386791094588615	No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT	37383	0.109149570883614	No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA	36931	0.107829837153325	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GAGCACA	228895	72.12893	9
AGAGCAC	232105	71.16206	8
ATCGGAA	235180	70.08059	2
CGGAAGA	235810	69.68188	4
GATCGGA	237490	69.37196	1
AAGAGCA	243890	67.94336	7
TCGGAAG	243445	67.55146	3
GAAGAGC	248215	66.58739	6
GGAAGAG	274500	60.320187	5
TCGTATG	161405	46.49672	42-43
TATGCCG	174580	45.988323	46-47
TATCTCG	165810	45.854015	38-39
GTATGCC	178925	45.473724	44-45
TCTCGTA	165655	45.409832	40-41
ATGCCGT	178720	45.278896	46-47
CGTATGC	163135	45.257748	44-45
GCCGTCT	176510	44.84414	48-49
ACCGATG	182220	44.728027	32-33
CACCGAT	185150	44.72775	30-31
CCGATGT	185095	44.591026	32-33