FastQCFastQC Report
Thu 8 Feb 2024
dm_104_treated_S12_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamedm_104_treated_S12_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31788172
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17414235.478210574675386No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA337870.10628796144679221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG56100.036.1539653
CAATCGC73750.028.4026344
TGGCGCG232000.024.9168723
AATCGCC93200.022.9849035
GTGGCGC297200.019.3866862
ATCAATC111800.018.7789822
GATCAAT125600.017.2073251
GGCGCGT391100.016.3591924
ATCGCCC139400.015.8101966
CGCGTGC406100.015.6847586
GCGTGCC384550.015.6002877
GGCGATC42950.015.1512692
GGGCGAT40750.014.8036131
CGTGCCT446200.013.5835328
GGTGGCG466450.012.7290651
GCGCGTG536900.012.075955
CGGAGGG111250.011.3999471
CGGTGGC399650.011.3861611
TAGTCCC439450.011.34372516-17
TCGCCCC197150.011.0103587
Produced by FastQC (version 0.11.4)