FastQCFastQC Report
Thu 8 Feb 2024
dm_104_treated_S12_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamedm_104_treated_S12_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31788172
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGGGGG6642032.0894658554131396TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGGTGG5288561.663687990614874TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGGGGG1049840.33026120533134146TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGGTGG1048390.3298050608257688TruSeq Adapter, Index 1 (97% over 45bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA544630.17133102211728313No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT418440.13163386683575262No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC361510.11372468979971545No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT348510.10963511837044294No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT320090.100694686061218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2171900.072.6732259
AGAGCAC2190600.072.098358
ATCGGAA2221600.070.905952
CGGAAGA2221700.070.805514
GATCGGA2242450.070.264521
TCGGAAG2291400.068.5803153
AAGAGCA2313350.068.5537957
GAAGAGC2330600.067.8651356
GGAAGAG2581850.061.3123865
ACGATCT1750050.045.25151436-37
TCACGAT1755100.045.16863634-35
CACGATC1766350.044.3609936-37
CATCACG1789150.044.28793332-33
TCTCGGG1447150.044.2290740-41
TCGGGTG632350.044.09818642-43
ATCACGA1787050.043.7900434-35
ATCTCGG1500450.043.67969540-41
GATCTCG1531500.043.3628338-39
CGATCTC1525450.043.19098738-39
CTCGGGT666050.042.1413242-43
Produced by FastQC (version 0.11.4)