FastQCFastQC Report
Thu 8 Feb 2024
dm_103_untreated_S9_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamedm_103_untreated_S9_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34130066
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19730225.780891252891219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG43250.029.872583
TGGCGCG233150.022.7973613
CAATCGC59850.022.142444
AATCGCC72050.019.1842355
GTGGCGC298700.018.0329912
GGCGCGT337750.017.2698464
CGCGTGC388350.014.9707526
ATCAATC103300.014.8062642
GCGTGCC391250.014.3863127
GGGCGAT52900.014.00744151
GGCGATC50400.013.9482942
GGTGGCG413700.013.20391
ATCGCCC108650.012.9403936
GCGCGTG454400.012.9305455
CGTGCCT446900.012.7012998
GATCAAT118200.012.6585231
CGGTGGC335600.010.8275311
GCCACGA100600.010.269484518-19
AGTGCGT97000.09.94064430-31
CTACTCG533650.09.7196726
Produced by FastQC (version 0.11.4)