FastQCFastQC Report
Thu 8 Feb 2024
dm_103_untreated_S9_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamedm_103_untreated_S9_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34130066
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGGGGG12026453.523711322445142TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGGTGG2960600.8674463155154754TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTGGGGGGG1203430.3526011347297131TruSeq Adapter, Index 4 (97% over 45bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA650460.19058269620691623No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT484640.1419979674226238No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT431180.126334358685389No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC410580.1202986246788975No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT387840.11363587752804229No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG380350.1114413315227694No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG347850.10191893563874152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2634050.068.16589
AGAGCAC2667450.067.3638848
CGGAAGA2724900.065.5378654
ATCGGAA2766700.064.717632
AAGAGCA2808600.064.177837
TCGGAAG2787150.064.060373
GATCGGA2800500.063.963281
GAAGAGC2838950.063.2576686
GGAAGAG3087200.058.2247625
AATCTCG1813900.045.13006638-39
TCTCGGG1807100.044.1901740-41
GACCAAT2059900.043.77282734-35
ACCAATC2079350.043.0312636-37
ATCTCGG1874250.042.86778340-41
CCAATCT2138500.042.2882636-37
ACTGACC2103500.042.2575532-33
TCGGGGG1522700.041.58373642-43
TGACCAA2144800.041.43044334-35
CACTGAC2203700.041.04475430-31
CTGACCA2217200.040.6653332-33
Produced by FastQC (version 0.11.4)