FastQCFastQC Report
Thu 8 Feb 2024
dm_103_treated_S10_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamedm_103_treated_S10_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28099132
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG333004311.85105290796883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG36650.033.8262333
CAATCGC47350.026.1821064
TGGCGCG168600.025.8344293
AATCGCC61000.021.80285
GTGGCGC211700.020.485052
GGCGCGT239150.019.8615724
CGCGTGC276650.017.3238546
GCGTGCC281750.016.2010617
ATCAATC84650.016.0481952
GGCGATC42150.015.776752
ATCGCCC89100.015.5664366
GGTGGCG294600.015.1075481
GCGCGTG325700.014.8024055
GATCAAT94450.014.7853791
CGTGCCT316350.014.564588
GGGCGAT42100.013.9902891
GGTCGAC17500.012.4854385
TCGCCCC116750.011.1475057
CGATCTG61150.010.6416294
GCCACGA77550.010.04491118-19
Produced by FastQC (version 0.11.4)