FastQCFastQC Report
Thu 8 Feb 2024
dm_103_treated_S10_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamedm_103_treated_S10_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28099132
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC21529177.661863007013882TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTATGC2703820.9622432465173657TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTGTGC2213680.7878108120919892TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGG667170.23743438053531332TruSeq Adapter, Index 3 (97% over 49bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA507550.1806283553527561No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATGC506810.18036500202212652TruSeq Adapter, Index 3 (98% over 50bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT448400.15957788304635176No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGGTGC421220.1499049863889034TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTGTGG376280.1339116097963453TruSeq Adapter, Index 3 (97% over 49bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC374500.13327813827131743No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT298100.10608868629821021No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTGC286010.10178606228832976TruSeq Adapter, Index 3 (98% over 50bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG285810.10171488571248392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA3733900.081.943589
AGAGCAC3750400.081.631168
CGGAAGA3750300.081.060154
ATCGGAA3767750.080.931722
GATCGGA3779150.080.700711
TCGGAAG3776100.080.526933
AAGAGCA3858650.079.512077
GAAGAGC3865050.079.093046
GGAAGAG4039000.075.698455
TCGTATG2429600.046.90532342-43
TATGCCG2629500.046.4214546-47
TCTCGTA2467300.046.39830840-41
ATGCCGT2616650.046.39001546-47
GTATGCC2666450.046.37717444-45
CGTATGC2377350.046.04714244-45
GCCGTCT2459650.045.9768948-49
CATCTCG2996000.045.82451238-39
ATCTCGT2809150.045.80832340-41
TGCCGTC2676650.045.4139848-49
CACTTAG3407450.045.3185430-31
Produced by FastQC (version 0.11.4)