FastQCFastQC Report
Thu 8 Feb 2024
dm_102_untreated_S7_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamedm_102_untreated_S7_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34173331
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG371970010.884803708482501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG46900.034.4347343
CAATCGC61550.026.4697044
TGGCGCG201400.024.6932283
AATCGCC77400.021.4788065
GTGGCGC256500.019.629612
GGCGCGT281400.019.1750854
ATCGCCC105800.016.1172836
ATCAATC107350.015.9734362
CGCGTGC341150.015.8306276
GATCAAT111950.015.1049431
GCGTGCC351800.014.797827
GGTGGCG350750.014.5174481
GGCGATC49250.013.9847582
GCGCGTG396950.013.9164015
GGGCGAT47500.013.6999871
CGTGCCT397750.013.2915318
TCGCCCC145150.011.5842867
CGGAGGG107000.010.6098021
CGATCAA28300.010.5741951
CGGTGGC283250.09.8102291
Produced by FastQC (version 0.11.4)