FastQCFastQC Report
Thu 8 Feb 2024
dm_102_untreated_S7_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamedm_102_untreated_S7_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34173331
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC30338688.87788199517337TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTGGTATGC2933160.8583184355075015TruSeq Adapter, Index 6 (98% over 50bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT594630.17400410864249669No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA523510.15319255825544192No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT448240.13116661059467688No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC444040.12993758202851224No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT407030.11910749935380897No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG348240.10190402568599473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA4261300.080.587279
AGAGCAC4289350.080.1045468
CGGAAGA4320300.079.039724
ATCGGAA4355700.078.7476962
TCGGAAG4362300.078.370053
GATCGGA4377850.078.3495561
AAGAGCA4418350.077.961297
GAAGAGC4438750.077.3634646
GGAAGAG4670550.073.494435
TCGTATG3354850.046.7905342-43
TATCTCG3395650.046.50578338-39
ATGCCGT3675800.046.32793446-47
TCTCGTA3396450.046.32643540-41
GTATGCC3694200.046.2976244-45
TATGCCG3647400.046.27874846-47
CGCCAAT3742950.046.23602732-33
GCCGTCT3650350.046.0209548-49
CGTATGC3370300.045.90876844-45
GTCACGC3796750.045.6127428-29
ATCTCGT3415300.045.58225640-41
Produced by FastQC (version 0.11.4)