FastQCFastQC Report
Thu 8 Feb 2024
dm_102_treated_S8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamedm_102_treated_S8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31543833
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18482445.85928793117818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG41900.030.0414963
TGGCGCG190900.022.1449033
CAATCGC58350.021.490544
AATCGCC70150.018.7558335
GTGGCGC249150.017.1391492
GGCGCGT290150.016.0267094
CGCGTGC325650.014.3671076
ATCAATC96400.014.1414942
GCGTGCC337900.013.2558547
GGCGATC44200.013.0032992
GATCAAT110700.012.9154421
ATCGCCC106800.012.4973896
GGGCGAT45700.012.3686181
GGTGGCG355700.012.1653591
GCGCGTG395850.012.0592315
CGTGCCT385150.011.9011958
CTACTCG459200.010.1369766
CGGTGGC296250.010.0691241
TACTCGG468700.010.0125857
GCCACGA82300.09.49402318-19
Produced by FastQC (version 0.11.4)