FastQCFastQC Report
Thu 8 Feb 2024
dm_102_treated_S8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamedm_102_treated_S8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31543833
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC8534882.7057206395937996TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGTATGC1978130.6271051460359939TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTGTGC1395540.44241294328434977TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGGATGC602590.19103258630617273TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGGGTGC562550.17833913843000626TruSeq Adapter, Index 5 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGG552330.1750992024336421TruSeq Adapter, Index 5 (97% over 49bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA516880.16386087258323995No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT448620.14222114351163348No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT378210.11989982320791516No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC372610.11812451581264712No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTGTGG361440.11458341159744283TruSeq Adapter, Index 5 (97% over 49bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT347800.11025927001325425No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGGGTGG347270.11009124984905924TruSeq Adapter, Index 5 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGTGTGC320170.10150003013267284TruSeq Adapter, Index 5 (97% over 46bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2386650.069.89439
AGAGCAC2416650.069.052188
CGGAAGA2461850.067.381524
ATCGGAA2494850.066.670292
TCGGAAG2517150.065.874173
GATCGGA2525500.065.842011
AAGAGCA2546100.065.7259457
GAAGAGC2577050.064.768986
GGAAGAG2809250.059.432375
TCGTATG973950.046.3970242-43
TATGCCG1131550.045.9715146-47
CGTATGC932550.045.31946644-45
TCTCGTA1007750.045.22236340-41
GTATGCC1176950.044.98258644-45
ATCTCGT1257250.044.95563540-41
ATGCCGT1106650.044.844246-47
GATCTCG1506350.043.6838-39
CCGTCGT213000.042.9075150-51
GCCGTCT820100.042.7854748-49
TGCCGTC1022950.042.61137848-49
Produced by FastQC (version 0.11.4)