FastQCFastQC Report
Thu 8 Feb 2024
control_204_untreated_S5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecontrol_204_untreated_S5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34266172
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22623256.60221106693797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG67350.033.5704733
CAATCGC87550.026.041594
AATCGCC111750.021.0822325
TGGCGCG152550.020.6749273
ATCAATC125700.018.705042
GATCAAT140850.016.3558671
ATCGCCC166500.014.8059246
GTGGCGC221900.014.470322
GGTCGAC28250.014.2916665
CGGAGGG126200.012.9475571
CGCGTGC376550.012.2359531
GGCGCGT320100.011.9600164
GCGTGCC347050.011.9210592
CGAGAAT96250.011.3505221
GGGTCGA31600.011.2734434
AGGGCGA136650.011.2620584
TCGCCCC217550.011.1569187
TACTCGG413550.011.1525357
TGTAGTC374350.010.98849
GGCGATC41700.010.8212212
Produced by FastQC (version 0.11.4)