FastQCFastQC Report
Thu 8 Feb 2024
control_204_untreated_S5_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecontrol_204_untreated_S5_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34266172
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGGGGG14289604.170176931347919TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGGTGG3098760.9043204475831149TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGGGGG1651800.482049760329225TruSeq Adapter, Index 8 (97% over 45bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT617580.18023022822625182No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC489180.1427588701766862No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGGTGG392600.11457363839765937TruSeq Adapter, Index 8 (97% over 45bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA352120.10276023828982124No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT349070.10187014761964072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2770150.075.336939
AGAGCAC2801700.074.522448
CGGAAGA2861400.072.431934
ATCGGAA2888200.071.994072
AAGAGCA2931650.071.390737
GATCGGA2928100.071.009811
TCGGAAG2937850.070.5831763
GAAGAGC2970350.070.294426
GGAAGAG3223200.064.7608955
AATCTCG2062950.045.46119738-39
CTCGCTA154750.045.112511
TCTCGGG2063500.044.44160540-41
TCGCTAT159150.043.7140352
ATCTCGG2114250.043.6464840-41
TTGAATC2424750.042.83067336-37
GAATCTC2175550.042.46138838-39
CGTCTGA2462950.042.28874616-17
ACGTCTG2491600.042.1301414-15
TGAATCT2492550.042.0550536-37
TCGGGGG1777950.041.8151742-43
Produced by FastQC (version 0.11.4)