FastQCFastQC Report
Thu 8 Feb 2024
control_204_treated_S6_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecontrol_204_treated_S6_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35379849
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24813467.013444291410062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG63400.032.14073
CAATCGC81600.025.0300964
TGGCGCG196200.020.675033
AATCGCC107150.019.6822625
ATCAATC119450.018.212432
GGTCGAC29050.016.023755
GATCAAT136050.015.4316451
GTGGCGC271800.015.2040172
GGCGCGT343950.013.7960384
GGCGATC44150.013.2331412
ATCGCCC166650.013.0540246
GGGTCGA34000.012.4335564
CGGAGGG137200.012.1518251
CGCGTGC453700.011.5791011
GCGTGCC418550.011.348572
AGGGCGA139050.010.8969244
TGTAGTC438300.010.7506849
GGGCGAT49500.010.6513911
CCTGTAG447800.010.4692917
TACTCGG495950.010.420187
Produced by FastQC (version 0.11.4)