FastQCFastQC Report
Thu 8 Feb 2024
control_204_treated_S6_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecontrol_204_treated_S6_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35379849
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC19645735.552802104949628TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGTATGC2081690.5883829521149172TruSeq Adapter, Index 7 (98% over 50bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT679510.19206130585803236No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC567260.16033420606176133No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA476700.13473771468046683No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG424720.12004573563895087No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT413340.11682921541016186No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG386030.10911013215460585No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT366670.10363809071090156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA3079350.073.902089
AGAGCAC3123550.072.888238
CGGAAGA3227450.070.191864
AAGAGCA3273200.069.734157
ATCGGAA3282950.069.2109152
GAAGAGC3322250.068.506016
TCGGAAG3318050.068.326973
GATCGGA3339300.068.059911
GGAAGAG3604200.063.128475
TCGTATG2168950.046.31829542-43
TATGCCG2387100.045.62139546-47
TCTCGTA2214450.045.5262840-41
GTATGCC2425450.045.47441544-45
ATGCCGT2412100.045.30991446-47
CGTATGC2180500.045.2911144-45
ATCTCGT2259700.044.8246740-41
GCCGTCT2352700.044.80965448-49
CTCGCTA184100.044.6479071
CATCTCG2324050.044.37185738-39
TGCCGTC2428700.044.1510748-49
Produced by FastQC (version 0.11.4)