FastQCFastQC Report
Thu 8 Feb 2024
control_203_untreated_S3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecontrol_203_untreated_S3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37343314
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25957756.951110445098687No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT432330.11577172824029491No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC381710.10221642353434406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG73050.034.0721553
CAATCGC99050.025.36794
TGGCGCG202400.021.074363
AATCGCC127450.020.0132585
ATCAATC137650.018.2544212
GATCAAT143400.017.522461
GTGGCGC278450.015.5914882
GCGTGCC410600.013.9629412
GGCGCGT353700.013.8857334
ATCGCCC193250.013.7888076
CGCGTGC445900.013.6458391
CTACTCG580500.013.0018466
TACTCGG586500.012.9417237
CGGAGGG153450.012.6603291
CGTGCCT461200.012.5340043
GGCGATC51350.012.3951832
CCTGTAG477600.012.0835717
TGTAGTC475850.012.00853359
ACTCGGG642950.011.8648638
CGAGAAT97750.011.856641
Produced by FastQC (version 0.11.4)