FastQCFastQC Report
Thu 8 Feb 2024
control_203_untreated_S3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecontrol_203_untreated_S3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37343314
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC19781625.297232056051587TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGTATGC2238670.5994834845134527TruSeq Adapter, Index 10 (98% over 50bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT951350.2547577860925787No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC658700.17639034393144645No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG471150.12616716341779416No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA463870.12421768459007147No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTGTGC454920.12182100388840691TruSeq Adapter, Index 10 (98% over 50bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT373570.10003664913081899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA3100350.076.2755669
AGAGCAC3141000.075.329238
CGGAAGA3192200.073.618554
ATCGGAA3220150.073.195982
AAGAGCA3274250.072.481057
GATCGGA3263600.072.210631
TCGGAAG3280150.071.675743
GAAGAGC3333300.070.9763266
GGAAGAG3582900.065.977495
TCGTATG2202100.046.2798242-43
TATCTCG2338500.045.7257938-39
TATGCCG2411950.045.6741846-47
TCTCGTA2249350.045.4595940-41
GTATGCC2461450.045.268644-45
ATGCCGT2437100.045.18141646-47
CGTATGC2213950.045.04022244-45
ATCTCGT2345250.044.6399440-41
GCCGTCT2330250.044.63775348-49
CACTAGC2676650.044.39651530-31
GTCACTA2696450.044.2086928-29
Produced by FastQC (version 0.11.4)