FastQCFastQC Report
Thu 8 Feb 2024
control_203_treated_S4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecontrol_203_treated_S4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36020470
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22974206.378095566215544No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC423160.11747764534999128No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT422060.11717226343798401No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT391850.10878536565458474No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT371470.10312747168485031No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC367270.1019614680208226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG59900.033.4636083
CAATCGC81950.025.0970064
TGGCGCG208600.023.2259273
AATCGCC101350.020.1524435
ATCAATC121550.017.3115982
GTGGCGC283950.017.2801232
GGCGCGT346450.015.780394
GATCAAT133800.015.3361371
CGCGTGC464050.014.8010281
GCGTGCC441000.014.6914332
ATCGCCC155600.013.9504926
GGGCGAT49500.013.6260991
GGCGATC51250.013.3461812
CGTGCCT490900.013.1593083
CCTGTAG507150.012.7937327
TGTAGTC515750.012.4886319
CGGAGGG134450.012.4003721
TACTCGG620900.011.8115977
CTACTCG617500.011.7920196
GGTGGCG424450.011.5041611
Produced by FastQC (version 0.11.4)