FastQCFastQC Report
Thu 8 Feb 2024
control_203_treated_S4_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecontrol_203_treated_S4_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36020470
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC3940371.0939252041963916TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTGTGC3048950.8464492551041116TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGGGTGC2970520.8246755247779942TruSeq Adapter, Index 9 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGGGTGG2450730.6803714665577656TruSeq Adapter, Index 9 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTGTGG1258030.3492541879658983TruSeq Adapter, Index 9 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGGATGC1249270.3468222374666405TruSeq Adapter, Index 9 (98% over 50bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1005610.27917736775783325No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGG827090.22961665963825567TruSeq Adapter, Index 9 (97% over 49bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC738980.20515556848647448No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGTATGC699340.19415071485741303TruSeq Adapter, Index 9 (98% over 50bp)
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT648600.18006428011627834No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT606270.1683126289023991No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG581680.1614859550694369No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA530130.1471746481930969No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGTGTGC507400.14086434741134693TruSeq Adapter, Index 9 (97% over 46bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG452300.12556748981898347No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG447430.12421548080855135No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT446790.12403780405974714No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGGATGG446050.12383236531894226TruSeq Adapter, Index 9 (97% over 49bp)
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA437800.12154200097888783No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGGTGC428740.11902676450362808TruSeq Adapter, Index 9 (97% over 45bp)
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC373290.10363273993926232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2908650.072.2351469
AGAGCAC2952000.071.1550758
AAGAGCA3087700.068.2354357
CGGAAGA3070100.068.0733264
ATCGGAA3145550.066.716782
GAAGAGC3149600.066.713556
TCGGAAG3167700.066.0911253
GATCGGA3211350.065.370951
GGAAGAG3425450.061.325895
CTCGCTA232550.049.2055931
TCGCTAT240900.047.439172
TCGTATG539000.045.6461442-43
TATGCCG509250.045.00472646-47
CGCTATG255650.044.5707553
CGATCAG2371900.044.38498732-33
GTCACGA2392650.044.19546528-29
CACGATC2401350.044.0837730-31
ACGATCA2378900.043.96706432-33
TCACGAT2388700.043.8502730-31
GATCTCG2044350.043.71148738-39
Produced by FastQC (version 0.11.4)