FastQCFastQC Report
Thu 8 Feb 2024
control_201_untreated_S1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecontrol_201_untreated_S1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31711086
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20342576.414971092443822No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT327230.10319104177006111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG55150.035.0536043
CAATCGC71950.027.4626794
AATCGCC99100.020.4181465
ATCAATC110000.019.3018672
TGGCGCG146850.019.277743
GATCAAT118800.016.872541
ATCGCCC151050.014.2134046
CGCGTGC310350.014.0499721
GGCGCGT231400.013.8759814
GTGGCGC212550.013.7658842
GCGTGCC303800.013.46169952
CTACTCG419800.012.9891236
TACTCGG428550.012.8680027
CGTGCCT344650.011.8385543
GGCGATC44600.011.82152
ACTCGGG471450.011.6873618
TCGCCCC186000.011.210687
TGTAGTC365950.011.12381659
CTCGGGA507750.010.9921529
GCTACTC523150.010.8498085
Produced by FastQC (version 0.11.4)